The National Water Quality Management Strategy (NWQMS) aims to protect Australia’s water resources through maintaining and improving water and sediment quality. Since the 1990s, the NWQMS has had significant infrastructure investment from both the private and public sectors, and all States and Territories. The Australian and New Zealand Governments’ Guidelines for Fresh and Marine Water Quality represent the seminal guidance for the NWQMS and are used by all jurisdictions to inform the management and regulation of industrial discharges and catchment-wide water and sediment quality stressors. The Guidelines emphasise the benefits of Multiple Lines of Evidence approaches that offer the opportunity to use new monitoring and assessment technologies, such as eDNA. The Guidelines also promote the adoption of cost-effective strategies to develop water/sediment quality objectives, for which omics technologies show promising potential. The Office of the Supervising Scientist has overseen the operations and rehabilitation of the Ranger uranium mine, Northern Territory, for over 40 years. The Office has used eDNA for 10+ years, which has unlocked knowledge about ecosystem components and processes that were previously not assessable. This presentation will demonstrate how the Office of the Supervising Scientist has used eDNA research to inform the Ranger mine’s water and sediment quality objectives. It will explore areas that eDNA can be used within the Guidelines and demonstrate how these technologies can vastly improve environmental outcomes.