This study aimed to investigate glyphosate concentrations and release in Australian wastewaters and wastewater treatment plants (WWTPs), as little is known about this topic in the Australian environment. Glyphosate and its main biodegradation product, aminomethylphosphonic acid (AMPA) highly polar nature has historically made detection and investigation challenging. The main aim of the study was to determine glyphosate concentrations levels in the Australian wastewaters and receiving environments in Australian WWTPs and calculating mass loads. Sub-aims included assessing the spatial and temporal trends of glyphosate and AMPA in WWTPs, determining the removal efficiency of WWTPs, and assessing the stability of glyphosate in WW samples over a specific time period