The tire particles have been generated by the friction between tire and road surface, suspended in the atmospheres, and then settled land and aquatic environment. A part of them achieves to sediments in the coastal areas, and is residue for long period. The tires are made from the rubber, antioxidant, compound and reinforcing agents, and others, and contained over 100 kinds of substances. Information for their toxicities to aquatic organisms is limited, and required to be revealed urgently. In the present study, the effects to P. barbicornis by the exposures of tire particles or their effluents into the sea water were evaluated with preparing 2 tires produced the different tiremakers. Additionally, we also prepare both a new and a used one of individual tires. Each tire particles settled to the bottom of glass vessels with the gradually increasing the particles for individual tires. In a glass vessel, an amphipod was kept, and exposed to the particles for 2 weeks. As a result, we observed that the each LC50 was 0.01 and 0.02 mg/mL for 2 new tire particles, respectively, while LC50s for used tires were 0.03 and 0.2 mg/L, respectively. Additionally, each effluent was also resulted 0.9 and 1.0 mg/mL for new, and 0.8 to 1.0 mg/L for used, respectively. The toxicity variations of particle derived from new tires depending on the exposure time tended to remain lower than those from used. However, LC50s derived from effluents varied similar with new and used with exposure time. These results suggest that the particle toxicities were reduced depending on the driving distances, however those of effluents did not depend on the distances. A tire has several layers made from many components, and, therefore, the several different layers were continuously emerged to the tire surface.