Abstract:
With the prevalence of influenza, the antiviral drug Tamiflu has been heavily used. The metabolites of Tamiflu, oseltamivir ethylester (OE) and oseltamivir carboxylate (OC) were detected in rivers, sewage treatment plants, and other freshwater environments. The study of the freshwater organisms exposure with OEOC mixed is insufficient. This study evaluated the accumulative capacity of Daphnia magna with OE exposure and OE OC mixed exposure. D. magna have separately exposed in two groups: OE single exposure and OEOC mixed exposure for 72 hours. We analyzed the time-course OC and OE concentrations in D. magna with a Thermo Finnigan Acella1250 ultra-high-pressure liquid chromatography (UHPLC) system linked with a Thermo Finnigan TSQ Quantum ultra-triple quadrupole mass spectrometer (MS/MS). The highest accumulated concentration of OE in the single OE group and OEOC mixed exposure group is 379.0 ng/g ww and 350.1 ng/g ww, and the highest OE concentration in the OE group is higher than in the OEOC group. The uptake rate and depuration rate of OE in the single OE group are 0.042 ml/g/hr and 0.008 ml/g/hr; and 0.032 ml/g/hr for uptake rate and 0.006 ml/g/hr for depuration rate in the OEOC group. During 72 hours, the uptake rate and depuration rate with OC in the OEOC group is 0.053 ml/g/hr and 0.014 ml/g/hr. In conclusion, the The water environment with only OE exposure can be detected the concentration of OC transferred from OE.